Liquid culture expansion vial – 20mL vial/10mL nutrient
Step by step instructions: (photos here)
1 . Swab the grey rubber centre portion and inject the liquid culture vial with approx 1 – 2 mL of culture/spores. Incubate at correct temp (usually 20-28 C).
Mix contents by swirling gently every few days. Within 1-2 weeks, white fluffy growth of mycelium will be visible in the liquid. The liquid above the fluffy mycelia should remain transparent – if it is cloudy even after being left to settle for a few minutes it may be contaminated with bacteria. If so, best to get another vial and start again.
2. This step needs to be done carefully, so get everything ready, read through first and have a practice run. Work in a clean area. Mix the contents of the vial by shaking. Attach the needle to the syringe (leaving the protective needle sheath in place). Swab the rubber injection port, remove the sheath from the needle and pierce through the centre of the injection port. Incline the vial so that the liquid reaches the underside of the rubber injection and fill most of the syringe. Don’t worry about getting it all. Carefully replace the sheath over the needle.
Try not to draw any extra air into the syringe. The still-moist alcohol swab can be placed over needle when withdrawing to reduce risk of contamination and clean the port.
3. Inoculate MycoFarm or MycoBox using the culture in the usual way.