Swab and inoculate 2-5mL through top injection port. The needle will pierce the internal bag containing nutrient solution – take care not to push needle right out through the back of the bags. Once inoculated keep upright (!).
Incubate at correct temperature. You should see white fuffy growth of mycelium within 7-14 days.
To withdraw culture, lean against a book or similar item to get a 45 degree angle, swab and withdraw. If taking multiple samples swab and make a new hole each time.