Liquid culture

Liquid culture jar

Oyster mushroom cultures or spores can be expanded in a liquid culture jar nutrient solution and, once growing, transferred to a MycoFarm or MycoBox. This leads to a more rapid colonisation and better yield in many of these species (especially species such as Pleurotus tuberregium). It is also a handy way to expand and store cultures for future use.

Where to buy?

Jars can be purchased here

N.B. This procedure should only be attempted by competent adults who are familiar with safe working lab practices. If in doubt, please do not purchase or attempt to use this product.

Kit contains: Jar with injection port and air filter, 4 x swabs and 3 x syringes to remove growing cultures.

1 . Swab the grey rubber centre portion and inject the liquid culture vial with approx 2 - 10 mL of culture/spores. Incubate at correct temp (usually 20-28 C).

Mix contents by swirling gently every few days. Within 1-2 weeks, white fluffy growth of mycelium will be visible in the liquid. The liquid above the fluffy mycelia should remain transparent – if it is cloudy even after being left to settle for a few minutes it may be contaminated with bacteria. If so, best to get another vial and start again.

2. This step needs to be done carefully, so get everything ready, read through first and have a practice run. Work in a clean area. Gently mix the contents of the jar. Attach the needle to the syringe (leaving the protective needle sheath in place). Swab the rubber injection port, remove the sheath from the needle and pierce through the centre of the injection port. Incline the jar so that the liquid reaches the underside of the rubber injection and fill the syringe. Carefully replace the sheath over the needle.

Try not to draw any extra air into the syringe. The still-moist alcohol swab can be placed over needle when withdrawing to reduce risk of contamination and clean the port.

3. Inoculate MycoFarm or MycoBox using the culture in the usual way.